DNA extraction and polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
A Simple and Rapid Leaf Genomic DNA Extraction Method for Polymerase Chain Reaction Analysis
In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...
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Rapid isolation of high-purity microbial genomic DNA is necessary for genome analysis. In this study, the authors compared a one-hour procedure using a microwave with enzymatic and boiling methods of genomic DNA extraction from Gram-negative and Gram-positive bacteria. High DNA concentration and purity were observed for both MRSA and ESBL strains (80.1 and 91.1 μg/ml; OD260/280, 1.82 and 1.70, ...
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ژورنال
عنوان ژورنال: Journal of Cytology
سال: 2019
ISSN: 0970-9371
DOI: 10.4103/joc.joc_110_18